Post-doctoral fellow, INSERM U1211, Johan Garaude’s team.
Title: Mitochondrial metabolism adaptations during Gram-negative and Gram-positive bacterial infections
Upon bacterial infection, macrophages engage profound metabolic rearrangements, which are required for bacteria clearance and inflammation. At the core of innate immune signaling pathways and metabolism are mitochondria which are a central cellular energy source and serve as signaling platforms for diverse innate immune receptors signaling. Hence, mitochondria are now defined as a rheostat of macrophages immune function. Catabolic pathways fuel mitochondria electron transport chain (ETC) which is composed of four complexes (CI, CII, CIII and CIV) found as free mobile units in the inner mitochondrial membrane or organized as supercomplex (SCs). However, the mechanisms by which bacteria recognition triggers mitochondrial electron transport chain (ETC) modifications and how this contributes to macrophage-mediated bacteria clearance is still poorly understood. In this project, I am evaluating the specificities of Gram-negative and Gram-positive bacteria-induced mitochondrial adaptations and the subsequent metabolism-linked bactericidal activity and cytokine production. This work will reveal specific metabolic rewiring triggered by Gram-negative and Gram-positive bacteria sensing, which can potential be targeted to manipulate innate immunity.