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23 March @ 13 h 30 min - 14 h 30 min UTC+1
Institut Interdisciplinaire de Neurosciences (IINS), CNRS, Université de Bordeaux, France.
Title of conference: “Multi-Protein printing by Light-Induced Molecular Adsorption: Application to cell biology.”
Abstract (pdf here: VincentStruderAbstract Immunoconcept )
Established protein patterning methods either rely on a slow serial writing process or are based on parallelized photolithographic techniques where cumbersome mask alignment procedures usually impair the ability to generate multi-protein patterns. Here we describe how to perform high resolution multi protein micro-patterning using Light Induced Molecular Adsorption of Proteins (LIMAP). LIMAP is based on a water-soluble photo-initiator which is able to reverse the antifouling property of polymer brushes when exposed to UV light. We show that the density of adsorbed proteins scales almost linearly with the dose of UV light. Moreover LIMAP is well adapted to wide field illumination schemes. Consequently, micron-scale adhesive patterns where proteins can adsorb, are printed within seconds. Protein patterns ranging from 500nm to 1 mm can be printed in one step and for a given fluorescent protein controlled gradients of arbitrary shape can be patterned. We show that the range of application extends from the single molecule up to the multicellular scale with an exquisite control over local protein density: micropatterns of individual fluorescent molecule provide a tool for the quantitative study of biomolecular interactions. At larger scale, the ability to rapidly generate complex and dynamic protein landscapes enable studies of the cell-cell and cell-matrix interactions. Altogether, LIMAP provides a generic method for fast high resolution patterning of multiple protein with applications to biomedical research and tissue engineering.
Team “Imagerie quantitative de la cellule”
Institut interdisciplinaire de Neurosciences
CNRS UMR 5297
Bâtiment de Génomique Fonctionnelle Bordeaux
Université Victor Segalen Bordeaux 2
146, rue Léo Saignat
More informations about Vincent Struder here.
Invited by Pr. Jean-François Moreau